Acid Phosphtase
An acid phosphatase (AP) test is a presumptive test that screens for the presence of semen in a sample:
How it works
The test uses an enzyme called acid phosphatase to react with a reagent to produce a purple color. A purple color within 30 seconds indicates a positive result, while no color change after 60 seconds indicates a negative result.
When it's used
The AP test is used in cases of sexual assault to screen exhibits for semen. It can be performed on a cut-out portion of a stain, an extract of a stain, or a wipe of the stained material.
Limitations
The AP test is not conclusive and can produce false positives or false negatives. For example, AP can break down in the presence of heat, moisture, or age, which can lead to little or no enzyme activity. Also, the amount of AP in the sample can limit the test's ability to produce a positive result.
- Availibility: Booking Open
- Categories : Pathology
- Test Code : Serum (1 ml)
An acid phosphatase (AP) test is a presumptive test that screens for the presence of semen in a sample:
How it works
The test uses an enzyme called acid phosphatase to react with a reagent to produce a purple color. A purple color within 30 seconds indicates a positive result, while no color change after 60 seconds indicates a negative result.
When it's used
The AP test is used in cases of sexual assault to screen exhibits for semen. It can be performed on a cut-out portion of a stain, an extract of a stain, or a wipe of the stained material.
Limitations
The AP test is not conclusive and can produce false positives or false negatives. For example, AP can break down in the presence of heat, moisture, or age, which can lead to little or no enzyme activity. Also, the amount of AP in the sample can limit the test's ability to produce a positive result.

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