Hemoglobin HPLC/Electrophoresis

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Hemoglobin HPLC/Electrophoresis is a blood test used to identify abnormal hemoglobin variants and diagnose inherited hemoglobin disorders like thalassemia and sickle cell disease.

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Test Details

Also Known As

Hemoglobin Variant Analysis, Hb Electrophoresis Test, Hemoglobinopathy Screening, Thalassemia Test, Hb HPLC Test

Sample Type

Blood

Gender

Male, Female

Age Group

All Ages

Pathology

Test Code

Hemoglobin HPLC (High-Performance Liquid Chromatography) or Electrophoresis is a specialized laboratory test that separates different types of hemoglobin present in red blood cells based on their charge or retention time.

This test accurately quantifies normal hemoglobin fractions (HbA, HbA2, HbF) and detects abnormal variants such as HbS, HbC, HbE, and HbD. It is considered the gold standard for diagnosing thalassemia syndromes, sickle cell disease, and other hemoglobinopathies.

HPLC offers precise numerical values, making it especially useful for screening carriers, antenatal testing, family studies, and newborn screening. Electrophoresis may be used as an alternative where HPLC is unavailable.

Recommended Tests

Frequently Asked Questions

It detects and measures normal and abnormal hemoglobin types in the blood.

No, fasting is not required.

HPLC provides precise identification and quantification of hemoglobin variants.

Yes, it is one of the most reliable tests for thalassemia screening.

Yes, transfusions within the last 3 months can alter results.

Yes, it is commonly recommended for antenatal screening.

Hemoglobin HPLC/Electrophoresis